Coding

Part:BBa_K4129008:Design

Designed by: Christian Wistrm   Group: iGEM22_DTU-Denmark   (2022-10-02)


Lactaldehyde oxidoreductase, reduction of furfural to furfuryl alcohol


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1068
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 943
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1068
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1068
    Illegal NgoMIV site found at 421
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 769


Design Notes

nucleotide sequence was codon optimized for Aspergillus niger using GenSmart's codon optimizer tool


Source

from Escherichia coli

References